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  • 產品名稱：Mouseanti-HumanIgEAntibody(FITC)

  • 產品型號：
  • 產品廠商：Acris-antibodies
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簡單介紹：
Mouseanti-HumanIgEAntibody(FITC)
詳情介紹：
Clone 4H10
Isotype IgG1
Cross-Reactivity (Details) Species reactivity (tested):Human
Background Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5?mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.Synonyms: Immunoglobulin E human
Research Area Immunology, Secondary Antibodies
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Excitation/Emission wavelength: 494 nm/514 nm
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2?% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Background publications Gould, Beavil, Vercelli: "IgE isotype determination: epsilon-germline gene transcription, DNA recombination and B-cell differentiation." in: British medical bulletin, Vol. 56, Issue 4, pp. 908-24, 2001 (PubMed).

Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

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