Buffer
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Nuclear extracts are supplied in dilution buffer (20 mM Hepes?pH 7.5, 400 mM NaCl, 20?% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 μM Na2MoO4, 1 mM NaVO3, 10?mM PNPP, 10 mM b-glycerophosphate, 1 mM DTT and protease inhibitors). Cells were cultured in medium supplemented with 5 μM Anisomycin for 1 hour at 37?°C immediately prior to harvesting.
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