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產(chǎn)品詳情

  • 產(chǎn)品名稱：MCF-7nuclearextract(H2O2post-treated)

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：ActiveMotif
  • 產(chǎn)品文檔：
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簡單介紹：
MCF-7nuclearextract(H2O2post-treated)
詳情介紹：
Characteristics

Origin: human breast carcinoma
Components 2 x 100 μg of MCF-7 nuclear extract (H2O2 post treated) at 2.50 μg/μl
Restrictions For Research Use only
Concentration 2.50 μg/μL
Buffer The MCF-7 nuclear extracts have been collected in Lysis Buffer. Post-collection treatment consists in exposing the nuclear extract to a final concentration of 200 mM H2O2 for 20?min on ice. The Lysis Buffer consists of 20?mM Hepes (?pH 7.9), 100 mM KCl, 1?mM MgCl2, 20?% glycerol, 0.5 mM PMSF and 0.5 mM DTT. The protein content has been determined by a Bradford-based assay.
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
Storage -80 °C
Storage Comment To ensure stability, extracts should be stored at -80°C.
Supplier Images
Activity Assay (AcA) image for MCF-7 nuclear extract (H<sub>2</sub>O<sub>2</sub> post-treated) (ABIN2669816) MCF-7 nuclear extract (H2O2 post-treated)
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