產(chǎn)品詳情
簡(jiǎn)單介紹:
HeLanuclearextract(TNF-αstimulated)
詳情介紹:
Characteristics |
Origin: Human Cervix Adenocarcinoma |
Components | 2 x 100 μg of HeLa nuclear extract (TNF-α stimulated) at 2.50 μg/μl |
Restrictions | For Research Use only |
Concentration | 2.50 μg/μL |
Buffer | The HeLa nuclear extracts have been collected in Lysis Buffer after a 30-minute incubation with TNF-α (20?ng/mL). The Lysis Buffer consists of 20 mM Hepes?pH 7.5, 350 mM NaCl, 20?% glycerol, 1?% Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA, 5 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay. |
Preservative | Dithiothreitol (DTT) |
Precaution of Use | This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles. |
Storage | -80 °C |
Storage Comment | To ensure stability, extracts should be stored at -80°C. |