產(chǎn)品詳情
簡單介紹:
WI-38nuclearextract
詳情介紹:
Characteristics |
Origin: human fibroblasts |
Components | 2 x 100 μg of WI-38 nuclear extract at 2.5 μg/μl |
Restrictions | For Research Use only |
Concentration | 2.5 μg/μL |
Buffer | The WI-38 nuclear extracts have been collected in Lysis Buffer. The Lysis Buffer consists of 20 mM Hepes?pH 7.5, 400 mM NaCl, 20?% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 μM Na2MoO4, 1 mM NaVO3, 10?mM PNPP, 10 mM b-glycerophosphate, 1 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay. |
Preservative | Dithiothreitol (DTT) |
Precaution of Use | This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles. |
Storage | -80 °C |
Storage Comment | To ensure stability, extracts should be stored at -80°C. |