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Characteristics
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Opti-Seal Real Time PCR sealing films are based on 50 μm polyolefin films with 50 μm inert encapsulated silicone adhesive. They are optically clear, with low auto-fluorescence, and are especially suited for real-time qPCR, storage, and protein crystallization applications. The encapsulated silicone adhesive is non-tacky until pressed against the sealing surface, at which time adhesive is released only in sealing areas to form the strongest available heat-resistant seal around each well on the plate. Adhesive on non-sealing areas of the film, such as directly over sample wells, remains encapsulated and inert. abm's Opti-Seal qPCR sealing films are sized to fit within the edges of raised-rim 96-well plates. Their consistent high optical clarity makes possible reproducible, reliable, and consistent DNA amplification measurements and crystal detection. Two end tabs assist in positioning the film on the plate, and the non-tacky adhesive surface simplifies handling. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with films that overlap the plate rim. Film's dimensions are 78.6 mm by 139.7 mm including the end tabs (length is 113.0 mm with the end tabs excluded).
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