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產(chǎn)品詳情

  • 產(chǎn)品名稱：HumanGrowthFactorArrayG1

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：RayBiotech
  • 產(chǎn)品文檔：
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HumanGrowthFactorArrayG1
詳情介紹：
Purpose G-Series Human Growth Factor Antibody Array 1 Kit. Detects 41 Human Growth Factors. Suitable for all liquid sample types.
Sample Type Plasma, Cell Culture Supernatant, Serum, Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Detection Method Fluorometric
Specificity Amphiregulin, bFGF, beta-NGF, EGF, EGFR, FGF-4, FGF-6, FGF-7 (KGF), GCSF, GDNF, GM-CSF, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, IGF-1 R, IGF-2, M-CSF, M-CSF R, NT-3, NT-4, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PLGF, SCF, SCF R (CD117/c-kit), TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, VEGF-A, VEGFR2, VEGFR3, VEGF-D
Characteristics
  • High sensitivity and specificity
  • Low sample volume (10-100?μL per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays
Components Cytokine Antibody Array glass slide (4 or 8 arrays per slide)
Biotinylated Detection Antibodies
Streptavidin-conjugated HiLytePlus? 555 Fluor
Blocking Buffer
20X Wash Buffer I
20X Wash Buffer II
2X Cell Lysis Buffer
G-Series Antibody Array accessories
Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film
Material not included Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner
Application Notes Completely cover array area with sample or buffer during incubation. Avoid foaming during incubation steps. Perform all incubation and wash steps under gentle rocking or rotation. Cover the incubation chamber with adhesive film during incubation, particularly when incubation is more than 2 hours or <70?μl 6="" 7="" 10="" 13="" of="" sample="" or="" reagent="" is="" used.="" several="" incubation="" steps="" such="" as="" step="" (blocking),="" (sample="" incubation),="" (detection="" antibody="" (cy3="" equivalent="" dyestreptavidin="" incubation)="" may="" be="" done="" overnight="" at="" 4?°c.="" please="" make="" sure="" to="" cover="" the="" chamber="" tightly="" prevent="" evaporation.="" <="" td="">
Comment

The G-Series arrays feature fluorescent signal detection. The antibodies are spotted on glass slide solid supports and require a laser scanner for data collection.
All G-Series arrays work on the sandwich ELISA principle, utilizing a matched pair of antibodies: an immobilized capture antibody and a corresponding biotinylated detection antibody.
Sample Volume 100 μL
Plate Glass Slide
Protocol
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
Restrictions For Research Use only
Handling Advice Do not touch the surface of the slides, as the microarray slides are very sensitive. Hold the slides by the edges only. Handle all buffers and slides with powder free gloves. Handle glass slide/s in clean environment. The G-Series slides do not have bar codes. To help distinguish one slide from another, transcribe the slide serial number from the slide bag to the back of the slide with a fine point permanent marker. Please write the number on the very bottom edge of the slide, taking care to avoid writing on the array well areas.
Storage -20 °C
Storage Comment For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Product cited in: Peloso, Petrosyan, Da Sacco, Booth, Zambon, O?Brien, Aardema, Robertson, De Filippo, Soker, Stratta, Perin, Orlando: "Renal Extracellular Matrix Scaffolds From Discarded Kidneys Maintain Glomerular Morphometry and Vascular Resilience and Retains Critical Growth Factors." in: Transplantation, Vol. 99, Issue 9, pp. 1807-16, 2015 (PubMed).

Zhang, Xu, Zhou, Zhang, Zhou, Zhao, Wang, Lu et al.: "The differentiation of human adipose-derived stem cells towards a urothelium-like phenotype in vitro and the dynamic temporal changes of related cytokines by both paracrine and autocrine signal ..." in: PLoS ONE, Vol. 9, Issue 4, pp. e95583, 2014 (PubMed).

Liu, Li, Zhang, Wan, Xi, Hu, Ding, Zhu, Wang, Wang, Feng, Wang, Ma, Gao: "SIX1 promotes tumor lymphangiogenesis by coordinating TGF? signals that increase expression of VEGF-C." in: Cancer research, Vol. 74, Issue 19, pp. 5597-607, 2014 (PubMed).

Balasubramanian, Venugopal, Sundarraj, Zakaria, Majumdar, Ta: "Comparison of chemokine and receptor gene expression between Wharton's jelly and bone marrow-derived mesenchymal stromal cells." in: Cytotherapy, Vol. 14, Issue 1, pp. 26-33, 2011 (PubMed).

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