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產(chǎn)品詳情

  • 產(chǎn)品名稱：GlycanArray100

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：RayBiotech
  • 產(chǎn)品文檔：
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GlycanArray100
詳情介紹：
Purpose Detection of binding partners for 100 different glycans in purified proteins, serum, plasma, cell culture supernatants, cell/tissue lysates or other body fluids.
Sample Type Plasma, Cell Culture Supernatant, Purified Protein, Serum, Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Detection Method Fluorometric
Specificity β-Glc-, β-Gal-, α-Man-, α-Fuc-, α-Rha-, β-GlcNAc-, β-GalNAc-, Tobramycin, Gal-β-1,3-GlcNAc-β-, Gal-α-1,3-Gal-β-1,3-GlcNAc-β-, Neu5Ac-α-2,3-Gal-β-1,3-GlcNAc-β-, Neu5Ac-α-2,6-Gal-β-1,3-GlcNAc-β-, Neu5Gc-α-2,3-Gal-β-1,3-GlcNAc-β-, Neu5Gc-α-2,6-Gal-β-1,3-GlcNAc-β-, Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [Lewis A], Gal-β-1,4-Glc-β-, Gal-α-1,3-Gal-β-1,4-Glc-β-, Gal-α-1,4-Gal-β-1,4-Glc-β-, GlcNAc-β-1,3-Gal-β-1,4-Glc-β-, GalNAc-β-1,3-Gal-β-1,4-Glc-β-, Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-, Neu5Ac-α-2,6-Gal-β-1,4-Glc-β-, Neu5Gc-α-2,3-Gal-β-1,4-Glc-β-, Neu5Ac-α-2,6-Gal-β-1,4-Glc-β-, Gal-β-1,4-(Fuc-α-1,3)-Glc-β-, GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-β-, GlcNAc-β-1,6-GlcNAc-β-, 4-P-GlcNAc-β-1,4-Man-β-, Glc-α-1,2-Gal-α-1,3-Glc-α-, Gal-β-1,3-GalNAc-α-, Gal-β-1,4-GlcNAc-β-, Gal-β-1,4 -(Fuc-α-1,3)-GlcNAc-β- [Lewis X], Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β- [Sialyl Lewis X], Neu5Ac-α-2,3-Gal-β-1,3 -(Fuc-α-1,4)-GlcNAc-β- [Sialyl Lewis A], Neu5Gc-α-2,3-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [Sialyl Lewis A], Gal-α-1,4-Gal-β-1,3-GlcNAc-β-, Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-β- [LNnT], GlcA-β-1,4-GlcNAc-α-1,4-GlcA-β-, GlcNAc-β-1,6-(Gal-β-1,3)-GalNAc-α-O-Ser, Neu5Ac-α-2,3Gal-β-1,4-(6S)GlcNAc-β-, GalNAc-β-1,4-GlcNAc-β-, Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal β-1,4-Glc-β-, Neu5Gc-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-, GalNAc-α-1,3-(Fuc-α-1,2)-Gal-β-1,4-Glc-β- [Blood A antigen tetrose], GlcNAc-β-1,2-Man-α-, Neu5Ac-α-2,3-Gal-β-, Gal-β-1,3 -GalNAc-β-1,3-Gal-β-, Glc-α-1,2-Gal-α-, Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β-1,3-Gal-β-, Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3)-Glc-β- [3-Sialyl-3-fucosyllactose/ F-SL], GlcNAc-β-1,4-GlcNAc-β-, β-D-GlcA-, Gal-β-1,4-(6S)GlNAc-β-, GlcNAc-α-1,3-(Glc-α-1,2-Glc-α-1,2)-Gal-α-1,3-Glc-α-, Gal-β-1,3-GalNAc-β-1,4-(Neu5Gc-α-2,3)-Gal-β-1,4-Glc-β-, Sisomicin Sulfate, GalNAc-α-1,3-(Fuc-α-1,2)-Gal-β- [Blood A antigen trisaccharide], Fuc-α-1,2-Gal-β-1,4-GlcNAc-β- [Blood H antigen trisaccharide], Gal-α-1,3-(Fuc-α-1,2)-Gal-β- [Blood B antigen trisaccharide], Fuc-α-1,2-Gal-β-1,3-GlcNAc-β-1,3-Gal-β-1,4-Glc-β- [LNFP I], Fuc-α-1,2-Gal-β-1,4-Glc-β- [Blood H antigen trisaccharide], Gal-α-1,3-(Fuc-α-1,2)-Gal-β-1,4-Glc-β- [Blood B antigen tetrasaccharide], (Fuc-α-1,2)-Gal-β-1,4-(Fuc-α-1,3)-GlcNAc-β- [ Lewis Y], (Fuc-α-1,2)-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β- [ Lewis B], Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β-1,3-Gal-β-1,4-(Fuc-α-1,4)-Glc-β- [Lewis A], Gal-β-1,3-GalNAc-β-, Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-, Neu5Ac-α-2,6-Gal-β-1,3-GalNAc-β-, Neu5Ac-α-2,6-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-, Neu5Ac-α-2,3-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-, Neu5Ac-α-2,6-(Neu5Ac-α-2,3)-Gal-β-1,3-GalNAc-β-, GalNAc-β-1,4-(Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β- [GM2], GalNAc-β-1,4-(Neu5Ac-α-2,8-Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β- [GD2], Gal-α-1,4-Gal-β-1,4-GlcNAc-β-, β-D-Rha-, Glc-α-1,4-Glc-β-, Glc-α-1,6-Glc-α-1,4-Glc-β-, Maltotriose-β-, Glc-α-1,6-Glc-α-1,6-Glc-β-, Maltotetraose-β-, GlcNAc-α-1,4-GlcA-β-1,4-GlcNAc-α1,4-GlcA-β-, Maltohexaose-β-, Maltoheptaose-β-, Acarbose-β-, D-pentamannuronic acid-β-, L-pentaguluronic acid-β-, D-cellose-β-, Gal-α-1,3-Gal-β-, β-1,4-Xylotetrose, Chitin-trisaccharide, KDN-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-, Neu5Ac-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-Glc-β-, Neu5Ac-α-2,8-Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-β-, Neu5Ac-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-Glc-, Gal-β-1,3-GalNAc-β-1,4-(Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β-, Gentamicin Sulfate, Kanamycin sulfate, Geneticin Disulfate Salt (G418), Neomycin trisulfate, SGP
Characteristics
  • High sensitivity and specificity
  • Low sample volume (20-100?μL per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 samples on each slide
  • Suitable for high-throughput assays
Components Dialysis Vials
Labeling Reagent
Stop Solution
Glycan Array Glass Slide Assembly
Sample Diluent
20X Wash Buffer I
20X Wash Buffer II
Cy3 equivalent dye-conjugated Streptavidin
Slide Washer/Dryer
Adhesive device sealer
Labeling Buffer
Floating Dialysis Rack
Material not included Detection antibodies of interest (For sandwich-based method only)
Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Distilled or de-ionized water
1.5?mL Polypropylene microcentrifuge tubes
Gene microarray scanner or similar laser fluorescence scanner
Small plastic or glass containers
KCl, NaCl, KH2PO4 and Na2HPO4 (For label-based method only)
Beaker, stir plate and stir bar
Application Notes This array will help researchers:
Identify the glycans binding partners in biological samples
Identify whether target proteins are carbohydrate binding proteins
Probe binding of viruses and whole cells to glycans1-3
Profile the substrate specificity of enzymes (glycosyltransferases, glycosidases, etc.)
Profile the inflammatory immune response
Plate Glass Slide
Protocol Dry the glass slideBlock array surfacesIncubate samples (samples need to be biotinylated for the label-based approach)For the the sandwich-based principle, incubate with a detection antibody cocktailFor the label-based principle, incubate the labeled-streptavidin.Incubate with Cy3 Equivalent Dye-StreptavidinDisassemble the glass slideScan with a gene microarray laser scannerPerform densitometry and analysis
Restrictions For Research Use only
Storage -20 °C
Storage Comment For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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