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  • 產(chǎn)品名稱：Insulin ELISA Kit(INS)

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Insulin ELISA Kit(INS)
詳情介紹：
Purpose This ELISA Kit applies to the invitro quantitative determination of Guinea pigINSconcentrations in serum, plasma and other biological fluids.
Sample Type Plasma, Serum, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This kit recognizes natural and recombinantGuinea pigINS. No significant cross-reactivity or interference between Guinea pigINS and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between Guinea pigINS and all the analogues.
Sensitivity 1.875 ng/mL
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
Sample Volume 100 μL
Plate Pre-coated
Protocol This ELISA Kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to INS. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for INSand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of INS.You can calculate the concentration of INSin the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
Restrictions For Research Use only
Storage Comment All the reagents in the kit should be stored according to the labels on vials.Unused wellsshould bereturned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal.Substrate Reagent shouldn't be kept at -20°C(Check!). Exposure of reagents to strong light should beavoided in the process of incubation and storage. All the taps of reagents should be tightened to preventevaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur. 6th Edition, revised in June, 2015 www.elabscience.com 3
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