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產(chǎn)品詳情

  • 產(chǎn)品名稱(chēng)：PseudomonasExotoxinA(PEA) ELISA Kit

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  • 產(chǎn)品廠(chǎng)商：Abbexa
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PseudomonasExotoxinA(PEA) ELISA Kit
詳情介紹：
Purpose For quantitative detection of PEA in Human serum, plasma or any biological fluids.
Sample Type Serum, Plasma
Analytical Method Quantitative
Detection Method Colorimetric
Sensitivity 0.94 ng/mL
Components 1. One 96-well plate pre-coated with anti-Human PEA antibody
2. Lyophilized Human PEA standards: 2 tubes
3. Sample / Standard diluent buffer: 20ml
4. Biotin conjugated anti-Human PEA antibody (Concentrated): 120 μl. Dilution: 1:100
5. Antibody diluent buffer: 10 ml
6. HRP Streptavidin Conjugate (SABC) (Concentrated): 120 μl. Dilution: 1:100
7. SABC diluent buffer: 10 ml
8. TMB substrate: 10 ml
9. Stop solution: 10 ml
10. Wash buffer (25X): 30 ml
Material not included 1. 37°C incubator
2. Microplate reader (wavelength: 450nm)
3. Pipettes and disposable pipette tips
4. Automated plate washer (optional)
5. ELISA shaker
6. 1.5 ml tubes
7. Plate cover
8. Absorbent filter papers
9. Plastic or glass container with volume greater than 1L
Alternative Name Pseudomonas Exotoxin A
Background The Pseudomonas exotoxin (or exotoxin A) is an exotoxin produced by Pseudomonas aeruginosa. It inhibits elongation factor-2. It does so by ADPribosylation of EF2. This then causes the elongation of polypeptides to cease. The mechanism of the toxin is similar to that of Diphtheria toxin. It has been investigated as a treatment for hepatitis B and cancer.
Plate Pre-coated
Protocol This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PEA antibody is pre-coated onto 96-well plates. Biotin conjugated anti-PEA monoclonal antibody is used as a detection antibody. The standards, test samples and biotin conjugated detection antibody are added to the wells and washed with wash buffer. HRP Streptavidin is added and unbound conjugates are washed away with wash buffer. TMB substrate is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue colour product that changes into yellow after adding stop solution. The density of yellow is proportional to the PEA amount of sample captured in plate. The O.D. absorbance is measured spectrophotometrically at 450nm in a microplate reader, and then the concentration of PEA can be calculated.
Restrictions For Research Use only
Storage 4 °C,-20 °C
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