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產(chǎn)品名稱:Ratanti-Mouse(Murine)IgM(Chainmu)Antibody(FITC)
產(chǎn)品型號:
產(chǎn)品廠商:Acris-antibodies
產(chǎn)品文檔:
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簡單介紹:
Ratanti-Mouse(Murine)IgM(Chainmu)Antibody(FITC)
詳情介紹:
Product details
Product details
Immunogen
Polyclonal and monoclonal mouse IgM. Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line.
Clone
LO-MM-9
Isotype
IgG2a
Purification
Affinity chromatography on mouse IgM/Sepharose 4B
Target details
Target details
Background
Synonyms: Immunoglobulin M mouse
Research Area
Secondary Antibodies, Immunology
Application Details
Application Details
Application Notes
Flow Cytometry: 5?μg/mL, use 5 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Comment
Excitation/Emission wavelength: 494 nm/514 nm
Restrictions
For Research Use only
Handling
Handling
Concentration
1.0 mg/mL
Buffer
PBS,?pH 7.2 containing 0.09?% Sodium Azide and 50?% Glycerol
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
References
References
Background publications
Bazin, Cormont, De Clercq: "Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum."
in:
Journal of immunological methods
, Vol. 71, Issue 1, pp. 9-16, 1984 (PubMed).
Bazin, Beckers, Querinjean: "Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c."
in:
European journal of immunology
, Vol. 4, Issue 1, pp. 44-8, 1974 (PubMed).
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