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Concentration
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2.50 μg/μL
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Buffer
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The MCF-7 nuclear extracts have been collected in Lysis Buffer. Post-collection treatment consists in exposing the nuclear extract to a final concentration of 200 mM H2O2 for 20?min on ice. The Lysis Buffer consists of 20?mM Hepes (?pH 7.9), 100 mM KCl, 1?mM MgCl2, 20?% glycerol, 0.5 mM PMSF and 0.5 mM DTT. The protein content has been determined by a Bradford-based assay.
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Preservative
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Dithiothreitol (DTT)
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Precaution of Use
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This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
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It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
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Storage
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-80 °C
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Storage Comment
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To ensure stability, extracts should be stored at -80°C.
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