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產(chǎn)品詳情

  • 產(chǎn)品名稱：HeLanuclearextract(TNF-αstimulated)

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：ActiveMotif
  • 產(chǎn)品文檔：
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HeLanuclearextract(TNF-αstimulated)
詳情介紹：
Characteristics

Origin: Human Cervix Adenocarcinoma
Components 2 x 100 μg of HeLa nuclear extract (TNF-α stimulated) at 2.50 μg/μl
Restrictions For Research Use only
Concentration 2.50 μg/μL
Buffer The HeLa nuclear extracts have been collected in Lysis Buffer after a 30-minute incubation with TNF-α (20?ng/mL). The Lysis Buffer consists of 20 mM Hepes?pH 7.5, 350 mM NaCl, 20?% glycerol, 1?% Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA, 5 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay.
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
Storage -80 °C
Storage Comment To ensure stability, extracts should be stored at -80°C.
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