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Characteristics
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1. Cell Lysis Buffer for Western and IP is a kind of lysis buffer which lyses cells under non-denatured condition. The end cells lysed by this buffer can be used for PAGE, Western Blot, Immunoprecipitation and Co-Immunoprecipitation. 2. This product contains: 20mM Tris (pH 7.5) , 150mM NaCl, 1% Triton X-100 and different kinds of inhibitors (sodium pyrophosphate, beta-glycerophosphate, EDTA, Na3VO4, leupeptin) , which can effectively inhibit the protein degradation and maintain the original protein interaction. 3. The concentration of the protein which lysed by this buffer can be determined by our BCA Protein Assay Kits (Catalog # abx90640, abx90641, abx90642, abx90643). For the high concentration of detergent, it is not recommended to use Bradford Protein Assay Kit.
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