久热精品人妻视频,人人妻人人妻人人av,亚洲无码天堂在线观看,澳门成AV人片在线观看无码,久久国产精品二区,亚洲无码免费观看视频

產(chǎn)品詳情
  • 產(chǎn)品名稱:RatInflammationArrayGS1

  • 產(chǎn)品型號:
  • 產(chǎn)品廠商:RayBiotech
  • 產(chǎn)品文檔:
你添加了1件商品 查看購物車
簡單介紹:
RatInflammationArrayGS1
詳情介紹:
Purpose G-Series Rat Inflammation Array 1 Kit. Semi-quantitative measurement of 10 rat cytokines. Suitable for all liquid sample types.
Sample Type Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Detection Method Fluorometric
Specificity IFN-gamma, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-4, IL-6, IL-10, IL-13, MCP-1 (CCL2), TNF alpha
Characteristics
  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 μL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation
Components Cytokine Antibody Array glass slide (4 or 8 arrays per slide)
Biotinylated Detection Antibodies
Streptavidin-conjugated HiLytePlus? 555 Fluor
Blocking Buffer
20X Wash Buffer I
20X Wash Buffer II
2X Cell Lysis Buffer
G-Series Antibody Array accessories
Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film
Material not included Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner
Application Notes Completely cover array area with sample or buffer during incubation. Avoid foaming during incubation steps. Perform all incubation and wash steps under gentle rocking or rotation. Cover the incubation chamber with adhesive film during incubation, particularly when incubation is more than 2 hours or <70?μl 6="" 7="" 10="" 13="" of="" sample="" or="" reagent="" is="" used.="" several="" incubation="" steps="" such="" as="" step="" (blocking),="" (sample="" incubation),="" (detection="" antibody="" (cy3="" equivalent="" dyestreptavidin="" incubation)="" may="" be="" done="" overnight="" at="" 4?°c.="" please="" make="" sure="" to="" cover="" the="" chamber="" tightly="" prevent="" evaporation.="" <="" td="">
Comment

The G-Series arrays feature fluorescent signal detection. The antibodies are spotted on glass slide solid supports and require a laser scanner for data collection.
All G-Series arrays work on the sandwich ELISA principle, utilizing a matched pair of antibodies: an immobilized capture antibody and a corresponding biotinylated detection antibody.

Sample Volume 100 μL
Plate Glass Slide
Protocol
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
Restrictions For Research Use only
Handling Advice Do not touch the surface of the slides, as the microarray slides are very sensitive. Hold the slides by the edges only. Handle all buffers and slides with powder free gloves. Handle glass slide/s in clean environment. The G-Series slides do not have bar codes. To help distinguish one slide from another, transcribe the slide serial number from the slide bag to the back of the slide with a fine point permanent marker. Please write the number on the very bottom edge of the slide, taking care to avoid writing on the array well areas.
Storage -20 °C
Storage Comment For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
田东县| 克山县| 乌兰察布市| 磐石市| 永新县| 嘉禾县| 永川市| 建湖县| 灵璧县| 通州区| 精河县| 永平县| 维西| 内江市| 淄博市| 舒兰市| 舟山市| 大田县| 莱西市| 新龙县| 南部县| 许昌市| 宝坻区| 武安市| 镇赉县| 吉木萨尔县| 石城县| 长宁区| 岫岩| 龙岩市| 德江县| 镇平县| 汉阴县| 栖霞市| 东城区| 乐业县| 正镶白旗| 留坝县| 丰都县| 紫阳县| 民丰县|