久热精品人妻视频,人人妻人人妻人人av,亚洲无码天堂在线观看,澳门成AV人片在线观看无码,久久国产精品二区,亚洲无码免费观看视频

產(chǎn)品詳情

  • 產(chǎn)品名稱：RatCytokineArrayGS3

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：RayBiotech
  • 產(chǎn)品文檔：
你添加了1件商品 查看購物車
簡單介紹：
RatCytokineArrayGS3
詳情介紹：
Purpose G-Series Rat Cytokine Array 3 Kit. Semi-quantitative measurement of 27 rat cytokines. Suitable for all liquid sample types.
Sample Type Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Detection Method Fluorometric
Specificity Beta-NGF, CD86 (B7-2), CINC-1 (CXCL1), CINC-2, CINC-3, CNTF, Fractalkine (CX3CL1), GM-CSF, ICAM-1 (CD54), IFN-gamma, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-10, IL-13, IL-2, IL-4, IL-6, LIX, L-Selectin (CD62L), MCP-1 (CCL2), PDGF-AA, Prolactin R, RAGE, TCK-1 (CXCL7), TIMP-1, TNF alpha, VEGF-A
Characteristics
  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 μL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation
Components Cytokine Antibody Array glass slide (4 or 8 arrays per slide)
Biotinylated Detection Antibodies
Streptavidin-conjugated HiLytePlus? 555 Fluor
Blocking Buffer
20X Wash Buffer I
20X Wash Buffer II
2X Cell Lysis Buffer
G-Series Antibody Array accessories
Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film
Material not included Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner
Application Notes Completely cover array area with sample or buffer during incubation. Avoid foaming during incubation steps. Perform all incubation and wash steps under gentle rocking or rotation. Cover the incubation chamber with adhesive film during incubation, particularly when incubation is more than 2 hours or <70?μl 6="" 7="" 10="" 13="" of="" sample="" or="" reagent="" is="" used.="" several="" incubation="" steps="" such="" as="" step="" (blocking),="" (sample="" incubation),="" (detection="" antibody="" (cy3="" equivalent="" dyestreptavidin="" incubation)="" may="" be="" done="" overnight="" at="" 4?°c.="" please="" make="" sure="" to="" cover="" the="" chamber="" tightly="" prevent="" evaporation.="" <="" td="">
Comment

The G-Series arrays feature fluorescent signal detection. The antibodies are spotted on glass slide solid supports and require a laser scanner for data collection.
All G-Series arrays work on the sandwich ELISA principle, utilizing a matched pair of antibodies: an immobilized capture antibody and a corresponding biotinylated detection antibody.
Sample Volume 100 μL
Plate Glass Slide
Protocol
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
Restrictions For Research Use only
Handling Advice Do not touch the surface of the slides, as the microarray slides are very sensitive. Hold the slides by the edges only. Handle all buffers and slides with powder free gloves. Handle glass slide/s in clean environment. The G-Series slides do not have bar codes. To help distinguish one slide from another, transcribe the slide serial number from the slide bag to the back of the slide with a fine point permanent marker. Please write the number on the very bottom edge of the slide, taking care to avoid writing on the array well areas.
Storage -20 °C
Storage Comment For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
略阳县| 宣城市| 盖州市| 新蔡县| 哈密市| 龙胜| 马边| 阿鲁科尔沁旗| 阿尔山市| 古丈县| 太仓市| 余庆县| 太原市| 界首市| 天气| 仪征市| 永吉县| 文山县| 定日县| 莱芜市| 惠安县| 西宁市| 莲花县| 金乡县| 荆门市| 梧州市| 郎溪县| 永州市| 富源县| 温宿县| 湖南省| 靖西县| 海宁市| 宣武区| 花垣县| 嫩江县| 双流县| 禹州市| 郑州市| 涪陵区| 稻城县|