產(chǎn)品詳情
簡單介紹:
HumanCytokineArrayQ19
詳情介紹:
Purpose | Quantibody? Human Cytokine Array 19 Kit. Quantitative measurement of 40 human cytokines. Suitable for all liquid sample types. |
Sample Type | Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell Lysate, Tissue Lysate |
Analytical Method | Quantitative |
Detection Method | Fluorometric |
Specificity | ADAM-22, Arylsulfatase B (ARSB), B3GNT2, CA5B, Caspase-7, Caspase-8, Integrin alpha-M (CD11b), SIRP-gamma (CD172g), CD39L2 (ENTPD6), CD39L4 (ENTPD5), Integrin alpha 2 (CD49b), CD7, CEACAM-3 (CD66d), Carboxypeptidase E (CPE), FABP6, FAM3C, GDF-3, GSTM1, Kallikrein 11, Kallikrein 12, Kremen-2, OSCAR, PTP1B, Reg3A, R-Spondin 2, S100 A13, Semaphorin 4C, Sirtuin 1, SMPD1, Sortilin, SPINK1, Stabilin-2, SULT2A1, Transcobalamin II, THSD1, TrkA, UCH-L3, VAP-A, vWF-A2, Wnt-4 |
Characteristics |
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Components |
Glass Chip with antibody arrays Sample Diluent Lyophilized protein standard mix Detection antibody cocktail Streptavidin-Fluorescent dye Wash buffer |
Material not included |
Distilled or deionized water Small plastic boxes or containers Pipettors, pipette tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Gene microarray scanner or similar laser fluorescence scanner |
Application Notes | Completely cover array area with sample or buffer during incubation. Avoid foaming during incubation steps. Perform all incubation and wash steps under gentle rocking or rotation. Cover the incubation chamber with adhesive film during incubation, particularly when incubation is more than 2 hours or <70?μl 6="" 7="" 10="" 13="" of="" sample="" or="" reagent="" is="" used.="" several="" incubation="" steps="" such="" as="" step="" (blocking),="" (sample="" incubation),="" (detection="" antibody="" (cy3="" equivalent="" dyestreptavidin="" incubation)="" may="" be="" done="" overnight="" at="" 4?°c.="" please="" make="" sure="" to="" cover="" the="" chamber="" tightly="" prevent="" evaporation.="" <="" td=""> 70?μl> |
Comment |
The Quantibody arrays are quantitative multiplex ELISA arrays featuring fluorescent detection. The antibodies are spotted on glass slide solid supports and require a laser scanner for data collection. Cytokine standards are provided with the array for calculation of target protein concentrations. |
Sample Volume | 100 μL |
Plate | Glass Slide |
Protocol |
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Restrictions | For Research Use only |
Handling Advice | Do not touch the surface of the slides, as the microarray slides are very sensitive. Hold the slides by the edges only. Handle all buffers and slides with powder free gloves. Handle glass slide/s in clean environment. The Quantibody slides do not have bar codes. To help distinguish one slide from another, transcribe the slide serial number from the slide bag to the back of the slide with an ultra-fine point permanent marker. Please Note:Red permanent marker can significantly interfere with fluorescent signal detection. We recommend marking your slides with a green, blue or black ultra-fine point permanent marker. Please write the number on the very bottom edge of the slide. Do not write on the arrayed well areas. |
Storage | -20 °C |
Storage Comment | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |