產(chǎn)品詳情
簡單介紹:
HumanCytokineArrayGS8000
詳情介紹:
Purpose | G-Series Human Cytokine Array 8000 Kit. A combination of 9 non-overlapping arrays to measure the relative expression levels of 360 human cytokines. Suitable for all liquid sample types. |
Sample Type | Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell Lysate, Tissue Lysate |
Analytical Method | Semi-Quantitative |
Detection Method | Fluorometric |
Specificity | 2B4 (CD244), 4-1BB (TNFRSF9/CD137), 6Ckine (CCL21), ACE-2, Activin A, ADAM-9, Adiponectin (ACRP30), Adipsin (Complement Factor D), aFGF (FGF-1), AgRP, Albumin, ALCAM (CD166), Alpha-fetoprotein, AMICA, Amphiregulin, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiostatin, ANGPTL3, ANGPTL4, APRIL, Axl, BAFF, BCAM, BCMA (TNFRSF17), BDNF, beta IG-H3, Beta-2 Microglobulin, Betacellulin (BTC), beta-NGF, bFGF, BLC (CXCL13), BMP-2, BMP-4, BMP-5, BMP-7, BMP-9, C5a, CA125, CA15-3, CA19-9, CA9, Cathepsin B, Cathepsin L, Cathepsin S, CCL28 (MEC), CD14, CD163, CD200, CD23, CD26 (DPPIV), CD30 (TNFRSF8), CD40 (TNFRSF5), CD40 Ligand (TNFSF5), CD80 (B7-1), CD97, CEA, CEACAM-1, Chemerin, CHI3L1, Clusterin, Coagulation Factor III (Tissue Factor/Thromboplastin), Cripto-1, CRP (C-Reactive Protein), CRTAM, CTACK (CCL27), CTLA-4 (CD152), CXCL14 (BRAK), CXCL16, Cystatin C, DAN, DcR3, Decorin, DKK-1, Dkk-3, Dkk-4, DLL1, DR6 (TNFRSF21), Dtk, E-Cadherin, EDA-A2, EGF, EGFR, EG-VEGF (PK1), ENA-78 (CXCL5), Endoglin (CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), ErbB2, ErbB3, Erythropoietin R, E-Selectin, FABP2, FAP, Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), Fc gamma RIIB/C (CD32B/C), Ferritin, Fetuin A, FGF-19, FGF-4, FGF-6, FGF-7 (KGF), FGF-9, Flt-3 Ligand, FOLH1 (PSMA), Follistatin, FOLR1, FSH, Furin, Galectin-3, Galectin-7, Gas 1, GASP-1 (WFIKKNRP), GASP-2 (WFIKKN), GCP-2 (CXCL6), GCSF, GCSF R (CD114), GDF-15, GDNF, GITR (TNFRSF18), GM-CSF, gp130, GRO alpha (CXCL1), GRO alpha/beta/gamma, Growth Hormone, HAI-2, HB-EGF, HCC-1 (CCL14), HCC-4 (CCL16), HCG beta, HGF, HGFR, HVEM (TNFRSF14), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), IFN-alpha / beta R2, IFN-gamma, IGF-1, IGF-1 R, IGF-2, IGF-2 R, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 F10 (IL-1HY2), IL-1 F5 (FIL1 delta), IL-1 F6 (FIL1 epsilon), IL-1 F7 (FIL1 zeta), IL-1 F8 (FIL1 eta), IL-1 F9 (IL-1 H1), IL-1 R1, IL-1 R2, IL-1 R4, IL-1 R5 (IL-18 R alpha), IL-1 R6 (IL-1 Rrp2), IL-1 ra (IL-1 F3), IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 2, IL-13 R1, IL-15, IL-16, IL-17 RA, IL-17 RB, IL-17A, IL-17B, IL-17C, IL-17F, IL-18, IL-18 BP alpha, IL-18 R beta (AcPL), IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-20, IL-21, IL-21 R, IL-23, IL-24, IL-27, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-3, IL-31, IL-33 (IL-1 F11), IL-34, IL-4, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-9, Insulin, IP-10 (CXCL10), I-TAC (CXCL11), Kallikrein 14, LAG-3, LAP/TGF beta 1, Layilin, LDL R, Legumain, Leptin, Leptin R, LIF, LIGHT (TNFSF14), LIMPII, Lipocalin-2 (NGAL), LOX-1, L-selectin (CD62L), Lymphotactin (XCL1), LYVE-1, Marapsin, MBL, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), Mer, MICA, MICB, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MMP-1, MMP-10, MMP-13, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MPIF-1 (CCL23), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NCAM-1 (CD56), Neprilysin, NGFR (TNFRSF16), Nidogen-1, Notch-1, NOV (CCN3), NrCAM, NRG1-beta 1 (HRG1-beta 1), NSE, NT-3, NT-4, Oncostatin M, Osteoactivin (GPNMB), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PAI-1, PARC (CCL18), P-Cadherin, PD-1, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PECAM-1 (CD31), Pepsinogen 1, PGRPs, Platelet Factor 4 (CXCL4), PLGF, Procalcitonin, Prolactin, Prostasin, PSA-free, RAGE, RANK (TNFRSF11A), RANTES (CCL5), RBP4, Resistin, SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), Serpin A4, sFRP-3, SIGIRR, Siglec-5 (CD170), Siglec-9, Sonic Hedgehog N-Terminal (Shh-N), SOST, ST2, Syndecan-1, TACE, TACI (TNFRSF13B), TARC (CCL17), TECK (CCL25), TFPI, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, TGF beta R3, Thrombomodulin, Thrombopoietin (TPO), Thrombospondin 1, Thyroglobulin, Tie-2, TIM-1 (KIM-1), TIMP-1, TIMP-2, TIMP-4, TLR2, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRAIL R1 (TNFRSF10A/DR4), TRAIL R2 (TNFRSF10B/DR5), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), Trance (TNFSF11), Transferrin, Trappin-2, TREM-1, TROP1 (EpCAM), Troponin I, TSH, TSLP, TWEAK (TNFSF12), uPA, uPAR, VCAM-1 (CD106), VE-Cadherin (CDH5), VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, VEGFR3, WIF-1, WISP-1 (CCN4), XEDAR |
Characteristics |
|
Components |
Cytokine Antibody Array glass slide (4 or 8 arrays per slide) Biotinylated Detection Antibodies Streptavidin-conjugated HiLytePlus? 555 Fluor Blocking Buffer 20X Wash Buffer I 20X Wash Buffer II 2X Cell Lysis Buffer G-Series Antibody Array accessories Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film |
Material not included |
Small plastic boxes or containers Pipettors, pipette tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Gene microarray scanner or similar laser fluorescence scanner |
Application Notes | Completely cover array area with sample or buffer during incubation. Avoid foaming during incubation steps. Perform all incubation and wash steps under gentle rocking or rotation. Cover the incubation chamber with adhesive film during incubation, particularly when incubation is more than 2 hours or <70?μl 6="" 7="" 10="" 13="" of="" sample="" or="" reagent="" is="" used.="" several="" incubation="" steps="" such="" as="" step="" (blocking),="" (sample="" incubation),="" (detection="" antibody="" (cy3="" equivalent="" dyestreptavidin="" incubation)="" may="" be="" done="" overnight="" at="" 4?°c.="" please="" make="" sure="" to="" cover="" the="" chamber="" tightly="" prevent="" evaporation.="" <="" td=""> 70?μl> |
Comment |
The G-Series arrays feature fluorescent signal detection. The antibodies are spotted on glass slide solid supports and require a laser scanner for data collection. |
Sample Volume | 100 μL |
Plate | Glass Slide |
Protocol |
|
Restrictions | For Research Use only |
Handling Advice | Do not touch the surface of the slides, as the microarray slides are very sensitive. Hold the slides by the edges only. Handle all buffers and slides with powder free gloves. Handle glass slide/s in clean environment. The G-Series slides do not have bar codes. To help distinguish one slide from another, transcribe the slide serial number from the slide bag to the back of the slide with a fine point permanent marker. Please write the number on the very bottom edge of the slide, taking care to avoid writing on the array well areas. |
Storage | -20 °C |
Storage Comment | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |