產品詳情
簡單介紹:
HeLaNuclearExtract
詳情介紹:
Characteristics | Nuclear extract is prepared from HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins. |
Application Notes | Western blot: 10-20 μg/lane HDAC Fluorometric Assay: 10 μg/assay HDAC Colorimetric Assay: 50 μg/assay |
Restrictions | For Research Use only |
Concentration | 5 mg/mL |
Storage | -80 °C |
Expiry Date | 12 months |
Background publications |
Dignam, Lebovitz, Roeder: "Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei." in: Nucleic acids research, Vol. 11, Issue 5, pp. 1475-89, 1983 (PubMed).
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