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Comment
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Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of modifying the proteins present in the extract. The best way to improve the yield of native proteins is to add inhibitors of these enzymes known to be present in the source material. This phosphatase inhibitor cocktail has been optimized and tested for tyrosine protein phosphatases, acid and alkaline phosphatase. 1. Reconstitute Phosphatase Inhibitor Cocktail Ⅱ with 1ml of water. 2. Add Phosphatase Inhibitor Cocktail Ⅱ solution into tissue or cell extracts in a ratio of 1:100. Optimal dilutions should be determined by end users.
Specificity: Tyrosine protein phosphatases, acid and alkaline phosphatases
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