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Comment
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Dissolve the inhibitor at 20 mM
in high purity (>99.9%) DMSO before use.
For use on intact cells:
1. Prepare desired concentrated stock solutions as follows:
5 mg Mu-F-HPh-FMK
in 55μl DMSO = 20 mM
in 1,10μl DMSO = 10 mM
in 2,20μl DMSO = 5 mM, etc.
2. Adding 2 μL of a 10 mM stock solution to 1 mL of culture medium gives a final Mu-F-HPh- FMK concentration of 20 μM.
Levels of DMSO above 0.2% may cause some cellular toxicity in culture medium, thus masking the effect of the protease inhibitor. For extended use in vitro or in vivo in animal studies: For experiments extending 12 to 48 hours, fresh inhibitor may have to be added (injected) due to inactivation of the inhibitor by endogenous cysteine proteases.
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