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Application Notes
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MM-Separators are intended for magnetic separation of MagSi beads from liquid samples for isolation and purification of nucleic acids and proteins, immunoprecipitation, immunoassays (ELISA), cell sorting, and purification of biomolecules. MM-Separators are designed for optimal magnetic bead separation in terms of speed and bead pellet positioning, as well as stability and user-friendliness.
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Comment
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The neodymium (NdFeB, NIB, or Neo) magnets used for the separators are the strongest permanent magnets made. These magnetic separators consists of a durable plexiglass block which is chemically inert and enables perfect visual control of the magnetic bead separation.
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Protocol
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MM-Separator M12 + 12 enables easy separation of beads from liquids for processing low amounts of samples with working volumes from 10 μL to 2 mL. In the centre of a stable block, 12 magnets allow accommodation of up to 24 centrifuge tubes.
The separator is designed to hold 2 mL microtubes in a stable upright position, and 1.5 mL tubes in a slightly tilted position for fast separation and easy handling in small-to-medium volumes. After liquids are pipetted off, microcentrifuge tubes can be conveniently placed on the outer positions for resuspending beads and further processing. MagSi-beads typically collect from a solution to the side of a centrifuge tube between 10 seconds and 3 minutes (depending on the type of beads and application). Separation of MagSi-DNA beads is completed within 10 seconds.
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Restrictions
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For Research Use only
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