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Sample Type
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Cell Extracts
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Specificity
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The Antibody with Clone 1H7 recognizes mTagBFP, mTagRFP, mTagRFP657 and mKate. Reactivity against other TagRFP derivatives not analyzed. Does not cross-react with common GFP- or dsRed/RFP derivatives including EBFP.
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Characteristics
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BFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4?% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. TagFP Selector thus features high affinity and superior capacity for TagFP fusion proteins while showing negligible non-specific background. TagFP Selector is compatible not only with physiological buffers but also with high stringency buffers. BFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
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Components
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4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody
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Material not included
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wash buffers, columns, tubes
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ProductDetails: Bead Ligand
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Antibody
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ProductDetails: Bead Matrix
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Agarose beads
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ProductDetails: Bead Size
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90 μm
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